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By Alice Cai Focus on PD
https://www.readbyqxmd.com/read/26997482/programmable-rna-tracking-in-live-cells-with-crispr-cas9
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David A Nelles, Mark Y Fang, Mitchell R O'Connell, Jia L Xu, Sebastian J Markmiller, Jennifer A Doudna, Gene W Yeo
RNA-programmed genome editing using CRISPR/Cas9 from Streptococcus pyogenes has enabled rapid and accessible alteration of specific genomic loci in many organisms. A flexible means to target RNA would allow alteration and imaging of endogenous RNA transcripts analogous to CRISPR/Cas-based genomic tools, but most RNA targeting methods rely on incorporation of exogenous tags. Here, we demonstrate that nuclease-inactive S. pyogenes CRISPR/Cas9 can bind RNA in a nucleic-acid-programmed manner and allow endogenous RNA tracking in living cells...
April 7, 2016: Cell
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