Gangqi Wang, Bram Heijs, Sarantos Kostidis, Ahmed Mahfouz, Rosalie G J Rietjens, Roel Bijkerk, Angela Koudijs, Loïs A K van der Pluijm, Cathelijne W van den Berg, Sébastien J Dumas, Peter Carmeliet, Martin Giera, Bernard M van den Berg, Ton J Rabelink
A common drawback of metabolic analyses of complex biological samples is the inability to consider cell-to-cell heterogeneity in the context of an organ or tissue. To overcome this limitation, we present an advanced high-spatial-resolution metabolomics approach using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) combined with isotope tracing. This method allows mapping of cell-type-specific dynamic changes in central carbon metabolism in the context of a complex heterogeneous tissue architecture, such as the kidney...
August 25, 2022: Nature metabolism