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8 papers 0 to 25 followers Artigos de caracterização de MVs em cultura celular
Sharad Kholia, Andrea Ranghino, Paolo Garnieri, Tatiana Lopatina, Maria Chiara Deregibus, Pietro Rispoli, Maria Felice Brizzi, Giovanni Camussi
Growing evidence suggests that small vesicles actively released from cells may encapsulate transcriptional regulators and RNA molecules. Their ability to interact with neighbouring cells and/or with distant cells through biological fluids, makes them a medium through which intercellular exchange of information can happen. Recently, membrane vesicles, which include exosomes and microvesicles, gained a place amongst the vast group of angiogenic mediators. In the present review we discuss the potential relevance of these vesicles in physiological and pathological situations of angiogenesis as well as their mechanism of action...
November 2016: Vascular Pharmacology
Yan Zhang, Mei Yu, Weidong Tian
Exosomes are nanovesicles that have emerged as a new intercellular communication system for transporting proteins and RNAs; recent studies have shown that they play a role in many physiological and pathological processes such as immune regulation, cell differentiation, infection and cancer. By transferring proteins, mRNAs and microRNAs, exosomes act as information vehicles that alter the behavior of recipient cells. Compared to direct cell-cell contact or secreted factors, exosomes can affect recipient cells in more efficient ways...
February 2016: Cell Proliferation
Katherine D Connolly, Irina A Guschina, Vincent Yeung, Aled Clayton, Mohd Shazli Draman, Christopher Von Ruhland, Marian Ludgate, Philip E James, D Aled Rees
Extracellular vesicles (EVs) are submicron vesicles released from many cell types, including adipocytes. EVs are implicated in the pathogenesis of obesity-driven cardiovascular disease, although the characteristics of adipocyte-derived EVs are not well described. We sought to define the characteristics of adipocyte-derived EVs before and after adipogenesis, hypothesising that adipogenesis would affect EV structure, molecular composition and function. Using 3T3-L1 cells, EVs were harvested at day 0 and day 15 of differentiation...
2015: Journal of Extracellular Vesicles
Heinrich J Huber, Paul Holvoet
PURPOSE OF REVIEW: Microvesicles, in general, and exosomes together with their delivered content in particular, are now being widely recognized as key players in atherosclerosis. We have previously reviewed the role of microvesicles in atherosclerosis pathogenesis, diagnosis and therapy. Here, we focus on the roles of exosomes and discuss their emergent role in mediating activation and response to inflammation, vessel infiltration and induction of coagulation. We will finally give an outlook to discuss novel detection techniques and systems biology based data analyses to investigate exosome-mediated cell-to-cell communication...
October 2015: Current Opinion in Lipidology
Jennifer A Muszynski, Justin Bale, Jyotsna Nateri, Kathleen Nicol, Yijie Wang, Valerie Wright, Clay B Marsh, Mikhail A Gavrilin, Anasuya Sarkar, Mark D Wewers, Mark W Hall
BACKGROUND: We have previously shown that critically ill children transfused with red blood cells (RBCs) of longer storage durations have more suppressed monocyte function after transfusion compared to children transfused with fresher RBCs and that older stored RBCs directly suppress monocyte function in vitro, through unknown mechanisms. We hypothesized that RBC-derived microvesicles (MVs) were responsible for monocyte suppression. STUDY DESIGN AND METHODS: To determine the role of stored RBC unit-derived MVs, we cocultured monocytes with supernatants, isolated MVs, or supernatants that had been depleted of MVs from prestorage leukoreduced RBCs that had been stored for either 7 or 30 days...
August 2015: Transfusion
Anne-lie Ståhl, Ida Arvidsson, Karl E Johansson, Milan Chromek, Johan Rebetz, Sebastian Loos, Ann-Charlotte Kristoffersson, Zivile D Békássy, Matthias Mörgelin, Diana Karpman
Shiga toxin (Stx) is the main virulence factor of enterohemorrhagic Escherichia coli, which are non-invasive strains that can lead to hemolytic uremic syndrome (HUS), associated with renal failure and death. Although bacteremia does not occur, bacterial virulence factors gain access to the circulation and are thereafter presumed to cause target organ damage. Stx was previously shown to circulate bound to blood cells but the mechanism by which it would potentially transfer to target organ cells has not been elucidated...
February 2015: PLoS Pathogens
Ali Danesh, Heather C Inglis, Rachael P Jackman, Shiquan Wu, Xutao Deng, Marcus O Muench, John W Heitman, Philip J Norris
Extracellular vesicles (EVs) are small, double membrane vesicles derived from leukocytes, platelets, and cells of other tissues under physiological or pathological conditions. Generation of EVs in stored blood is thought to be associated with adverse effects and potentially immunosuppression in blood transfusion recipients. We measured the quantity and cells of origin for EVs isolated from stored red blood cell (RBC) units and tested whether they had any effects on T-cell-mediated immune responses. Mixing peripheral blood mononuclear cells (PBMCs) with EVs resulted in secretion of proinflammatory cytokines and chemokines and increased survival of unstimulated PBMCs...
January 30, 2014: Blood
Arnon Blum
The tendency of sickle cells to adhere to the endothelium reflects the surface features not only of the red cells but also of the endothelial cells. Sickle cell disease is a prototype of a condition where the erythrocyte is under stress, ischemic, oxidative, or shear stress, that causes changes in the erythrocyte morphology. This change leads eventually to enhanced erythrocyte-endothelial cell adhesion. Reactive oxygen species generated by cytokine-activated inflammatory cells oxidize lipoproteins such as LDL and lipoprotein(a) within the vessel wall, facilitating uptake of these particles by activated macrophages and smooth muscle cells, with conversion into lipid-laden foam cells...
March 2009: European Journal of Internal Medicine
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