John McCullough, Amy K Clippinger, Nathaniel Talledge, Michael L Skowyra, Marissa G Saunders, Teresa V Naismith, Leremy A Colf, Pavel Afonine, Christopher Arthur, Wesley I Sundquist, Phyllis I Hanson, Adam Frost
The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1)...
December 18, 2015: Science