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Renal Biopsy

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42 papers 25 to 100 followers
By Isabel Acosta-Ochoa Nephrology senior staff. Valladolid. Spain
V Brkovic, M Milinkovic, M Kravljaca, M Lausevic, G Basta-Jovanovic, J Marković-Lipkovski, R Naumovic
Biopsy registries are one of the most important sources of accurate epidemiological data and the clinical presentation of renal diseases. A detailed analysis of clinicopathologic correlations over a period of 20 years (1987-2006) was performed earlier by our centre. The aim of this study was to check the current state and to register possible changes in clinicopathologic findings recorded under better socioeconomical circumstances and new management. Records of 665 renal biopsies performed at our institution were prospectively followed from 2007 to 2014...
October 2018: Pathology, Research and Practice
Sanjeev Sethi, Fernando C Fervenza
A kidney biopsy is done to determine the etiology of the glomerulonephritis (GN) and the severity of the lesion, to identify whether other lesions, related to or not related to the GN, are present on the kidney biopsy and finally to ascertain the extent of chronicity of the GN. The etiology of GN is based on the classification of GN into five groups: immune complex-mediated GN, antineutrophil cytoplasmic antibody (ANCA)-associated GN, anti-glomerular basement membrane (GBM) GN, monoclonal immunoglobulin-mediated GN and C3 glomerulopathy...
August 13, 2018: Nephrology, Dialysis, Transplantation
Gunnar Schley, Jutta Jordan, Stephan Ellmann, Seymour Rosen, Kai-Uwe Eckardt, Michael Uder, Carsten Willam, Tobias Bäuerle
OBJECTIVES: In human chronic kidney disease (CKD) the extent of renal tubulointerstitial fibrosis correlates with progressive loss of renal function. However, fibrosis can so far only be assessed by histology of kidney biopsies. Magnetic resonance imaging (MRI) can provide information about tissue architecture, but its potential to assess fibrosis and inflammation in diseased kidneys remains poorly defined. MATERIALS AND METHODS: We evaluated excised kidneys in a murine adenine-induced nephropathy model for CKD by MRI and correlated quantitative MRI parameters (T1, T2, and T2* relaxation times, apparent diffusion coefficient and fractional anisotropy) with histological hallmarks of progressive CKD, including renal fibrosis, inflammation, and microvascular rarefaction...
2018: PloS One
Kimberly A Muczynski, Nicolae Leca, Arthur E Anderson, Niamh Kieran, Susan K Anderson
Introduction: Current processing of renal biopsy samples provides limited information about immune mechanisms causing kidney injury and disease activity. We used flow cytometry with transplanted kidney biopsy samples to provide more information on the immune status of the kidney. Methods: To enhance the information available from a biopsy, we developed a technique for reducing a fraction of a renal biopsy sample to single cells for multicolor flow cytometry and quantitation of secreted cytokines present within the biopsy sample...
July 2018: KI Reports
German Ferrer, Nicole K Andeen, Joseph Lockridge, Douglas Norman, Bryan R Foster, Donald C Houghton, Megan L Troxell
There are differences in renal biopsy yield related to on-site evaluation, tissue division, and operator, among others. To understand these variations, we collected adequacy-associated data (%cortex, glomeruli, arteries, length) from consecutive native and allograft kidney biopsies over a 22-month period. In total, 1332 biopsies (native: 873, allograft: 459) were included, 617 obtained by nephrologists, 663 by radiologists, and 559 with access to on-site division. Proceduralists with access to on-site evaluation had significantly lower inadequacy rates and better division of tissue for light microscopy (LM), immunofluorescence, and electron microscopy than those without access to on-site evaluation...
June 5, 2018: American Journal of Surgical Pathology
Seth Winfree, Pierre C Dagher, Kenneth W Dunn, Michael T Eadon, Michael Ferkowicz, Daria Barwinska, Katherine J Kelly, Timothy A Sutton, Tarek M El-Achkar
Kidney biopsy remains the gold standard for uncovering the pathogenesis of acute and chronic kidney diseases. However, the ability to perform high resolution, quantitative, molecular and cellular interrogation of this precious tissue is still at a developing stage compared to other fields such as oncology. Here, we discuss recent advances in performing large-scale, three-dimensional (3D), multi-fluorescence imaging of kidney biopsies and quantitative analysis referred to as 3D tissue cytometry. This approach allows the accurate measurement of specific cell types and their spatial distribution in a thick section spanning the entire length of the biopsy...
June 5, 2018: Nephron
Anand Srivastava, Ragnar Palsson, Arnaud D Kaze, Margaret E Chen, Polly Palacios, Venkata Sabbisetti, Rebecca A Betensky, Theodore I Steinman, Ravi I Thadhani, Gearoid M McMahon, Isaac E Stillman, Helmut G Rennke, Sushrut S Waikar
Background Few studies have evaluated whether histopathologic lesions on kidney biopsy provide prognostic information beyond clinical and laboratory data. Methods We enrolled 676 individuals undergoing native kidney biopsy at three tertiary care hospitals into a prospective, observational cohort study. Biopsy specimens were adjudicated for semiquantitative scores in 13 categories of histopathology by two experienced renal pathologists. Proportional hazards models tested the association between histopathologic lesions and risk of kidney disease progression (≥40% eGFR decline or RRT)...
August 2018: Journal of the American Society of Nephrology: JASN
Kumar Sharma, Ljiljana Paša-Tolić
The road to precision medicine for nephrology is approaching quickly. In the present volume, the glomerular proteome has now been characterized at a single glomerulus level in mouse and human kidneys. Using the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) approach the authors demonstrated that LAMP1 is a key lysosomal protein that is increased in glomerular diseases and may play a pathogenic role.
June 2018: Kidney International
Jeffrey B Kopp
With normal aging, there are increased numbers of globally sclerotic glomeruli. Global glomerulosclerosis is also a feature of chronic kidney disease. In this issue, Hommos and colleagues provide data on the fraction of globally sclerotic glomeruli in healthy kidney donor biopsies. This provides reference for evaluation of whether isolated global glomerulosclerosis is evidence of pathology.
May 2018: Kidney International
Luisa Santangelo, Giuseppe Stefano Netti, Paolo Giordano, Vincenza Carbone, Marida Martino, Diletta Domenica Torres, Michele Rossini, Anna Maria Di Palma, Loreto Gesualdo, Mario Giordano
BACKGROUND: This study was conducted to investigate retrospectively the indications for renal biopsy (RB) in native kidneys and to analyze pathological findings in a single tertiary pediatric hospital in Southern Italy for the last 36 years. METHODS: All patients who underwent RB at our hospital from 1979 to 2014 were included. All renal tissue specimens were studied under light and immunofluorescent microscopy, while electron microscopy was performed only for specific clinical indications...
April 2018: World Journal of Pediatrics: WJP
Andrew F Malone, Haojia Wu, Benjamin D Humphreys
The renal biopsy provides critical diagnostic and prognostic information to clinicians including cases of acute kidney injury, chronic kidney disease, and allograft dysfunction. Today, biopsy specimens are read using a combination of light microscopy, electron microscopy, and indirect immunofluorescence, with a limited number of antibodies. These techniques all were perfected decades ago with only incremental changes since then. By contrast, recent advances in single-cell genomics are transforming scientists' ability to characterize cells...
January 2018: Seminars in Nephrology
R Haridian Sosa-Barrios, Victor Burguera, Nuria Rodriguez-Mendiola, Cristina Galeano, Sandra Elias, Gloria Ruiz-Roso, Sara Jimenez-Alvaro, Fernando Liaño, Maite Rivera-Gorrin
BACKGROUND: Percutaneous renal biopsy (PRB) is an important technique providing relevant information to guide diagnosis and treatment in renal disease. As an invasive procedure it has complications. Most studies up to date have analysed complications related to bleeding. We report the largest single-center experience on routine Doppler ultrasound (US) assessment post PRB, showing incidence and natural history of arteriovenous fistulae (AVF) post PRB. METHODS: We retrospectively analysed 327 consecutive adult PRB performed at Ramon Cajal University Hospital between January 2011 and December 2014...
December 20, 2017: BMC Nephrology
Adrian Ovidiu Văduva, Cosmin Glămeanu, Romeo Negrea, Mirela Danina Muntean, Alis Liliana Carmen Dema
Oxidative stress is a culprit responsible for the development of acute and chronic kidney diseases. We aimed to establish a working model for the dynamic in vivo assessment of reactive oxygen species (ROS) production in rat kidney. A randomized controlled study was performed in 36 adult male Wistar rats subjected to unilateral urinary obstruction (UUO) via ureteral ligation and compared to SHAM controls. Dihydroethidium (DHE) was injected in the femoral vein and in vivo confocal microscopy was performed in the 2nd, 6th and 10th day, respectively after surgery...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
Pedro Magalhães, Martin Pejchinovski, Katerina Markoska, Miroslaw Banasik, Marian Klinger, Dominika Švec-Billá, Ivan Rychlík, Merita Rroji, Arianna Restivo, Giovambattista Capasso, Flaviu Bob, Adalbert Schiller, Alberto Ortiz, Maria Vanessa Perez-Gomez, Pablo Cannata, Maria Dolores Sanchez-Niño, Radomir Naumovic, Voin Brkovic, Momir Polenakovic, William Mullen, Antonia Vlahou, Petra Zürbig, Lars Pape, Franco Ferrario, Colette Denis, Goce Spasovski, Harald Mischak, Joost P Schanstra
Chronic kidney disease (CKD) is a prevalent cause of morbidity and mortality worldwide. A hallmark of CKD progression is renal fibrosis characterized by excessive accumulation of extracellular matrix (ECM) proteins. In this study, we aimed to investigate the correlation of the urinary proteome classifier CKD273 and individual urinary peptides with the degree of fibrosis. In total, 42 kidney biopsies and urine samples were examined. The percentage of fibrosis per total tissue area was assessed in Masson trichrome stained kidney tissues...
December 5, 2017: Scientific Reports
Yvette Feldmann, Klaas Böer, Gunter Wolf, Martin Busch
BACKGROUND AND AIMS: Percutaneous renal biopsy is a necessary tool to diagnose renal diseases but complications may still occur. The aims of this study were to detect the rate of complications, possible risk factors and to identify the necessity of routine post-biopsy controls at 4 - 6, and 24 hours. MATERIALS AND METHODS: This single-center retrospective study included 500 consecutive biopsies of native and allograft kidneys performed at the Jena University Hospital...
April 2018: Clinical Nephrology
Francesco Paolo Schena, Ionut Nistor, Claudia Curci
Background: The diagnosis of glomerular diseases is based on the evaluation of histological lesions in renal tissue by means of light and electronic microscopy, and immunofluorescence technique. Frozen and archival formalin-fixed paraffin-embedded kidney biopsies represent a stored resource for high-throughput technologies. Transcriptomics makes it possible to study the whole gene-expression profile of cells and tissues in a specific period and/or condition. The results, whether considered alone or integrated with other omics data, could help to improve existing knowledge about the pathogenetic mechanisms of glomerulopathies...
July 1, 2018: Nephrology, Dialysis, Transplantation
Francesco Paolo Schena, Ionut Nistor, Claudia Curci
No abstract text is available yet for this article.
October 1, 2017: Nephrology, Dialysis, Transplantation
Laura Barisoni, Jeffrey B Hodgin
PURPOSE OF REVIEW: In this review, we will discuss (i) how the recent advancements in digital technology and computational engineering are currently applied to nephropathology in the setting of clinical research, trials, and practice; (ii) the benefits of the new digital environment; (iii) how recognizing its challenges provides opportunities for transformation; and (iv) nephropathology in the upcoming era of kidney precision and predictive medicine. RECENT FINDINGS: Recent studies highlighted how new standardized protocols facilitate the harmonization of digital pathology database infrastructure and morphologic, morphometric, and computer-aided quantitative analyses...
November 2017: Current Opinion in Nephrology and Hypertension
Maja T Lindenmeyer, Matthias Kretzler
Chronic kidney disease has severe impacts on the patient and represents a major burden to the health care systems worldwide. Despite an increased knowledge of pathophysiological processes involved in kidney diseases, the progress in defining novel treatment strategies has been limited. One reason is the descriptive disease categorization used in nephrology based on clinical findings or histopathological categories irrespective of potential different molecular disease mechanisms. To accelerate progress toward a targeted treatment, a definition of human disease extending from phenotypic disease classification to mechanism-based disease definitions is needed...
August 2017: Pflügers Archiv: European Journal of Physiology
Yoichiro Chikamatsu, Ken Matsuda, Yoichi Takeuchi, Saeko Kagaya, Yoshie Ojima, Hirotaka Fukami, Hiroyuki Sato, Ayako Saito, Yoshitsugu Iwakura, Tasuku Nagasawa
Background: The aim of this study was to investigate specific bleeding volume after percutaneous renal biopsy (PRB) and the correlation between bleeding volume and clinical parameters. Methods: A retrospective study of 252 consecutive patients (153 male patients and 99 female patients) who underwent PRB at the Department of Nephrology, Japanese Red Cross Ishinomaki Hospital, between July 2013 and January 2016 was conducted. PRB was performed under ultrasound guidance using an automated spring-loaded biopsy device and a 16-cm, 16-gauge needle...
February 2017: Clinical Kidney Journal
2017-07-03 16:55:18
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