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Nuclear DAGLa

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Gontzal García del Caño, Mario Montaña, Xabier Aretxabala, Imanol González-Burguera, Maider López de Jesús, Sergio Barrondo, Joan Sallés
Phosphoinositide (PtdIns) signaling involves the generation of lipid second messengers in response to stimuli in a receptor-mediated manner at the plasma membrane. In neuronal cells of adult brain, the standard model proposes that activation of metabotropic receptors coupled to Phospholipase C-β1 (PLC-β1) is linked to endocannabinoid signaling through the production of diacylglycerol (DAG), which could be systematically metabolized by 1,2-diacylglycerol Lipases (DAGL) to produce an increase of 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in the brain...
January 2014: Advances in Biological Regulation
Gontzal García del Caño, Xabier Aretxabala, Imanol González-Burguera, Mario Montaña, Maider López de Jesús, Sergio Barrondo, Ramón J Barrio, Carmen Sampedro, M Arantzazu Goicolea, Joan Sallés
In this report, we describe the localization of diacylglycerol lipase-α (DAGLα) in nuclei from adult cortical neurons, as assessed by double-immunofluorescence staining of rat brain cortical sections and purified intact nuclei and by western blot analysis of subnuclear fractions. Double-labeling assays using the anti-DAGLα antibody and NeuN combined with Hoechst staining showed that only nuclei of neuronal origin were DAGLα positive. At high resolution, DAGLα-signal displayed a punctate pattern in nuclear subdomains poor in Hoechst's chromatin and lamin B1 staining...
March 2015: Journal of Neurochemistry
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