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gamma secretase

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21 papers 0 to 25 followers
https://www.readbyqxmd.com/read/28230986/design-synthesis-and-evaluation-of-a-novel-series-of-oxadiazine-gamma-secretase-modulators-for-familial-alzheimer-s-disease
#1
Matthew G Bursavich, Bryce A Harrison, Raksha Acharya, Donald E Costa, Emily A Freeman, Hilliary E Hodgdon, Lori A Hrdlicka, Hong Jin, Sudarshan Kapadnis, Jeffrey S Moffit, Deirdre A Murphy, Scott Nolan, Holger Patzke, Cuyue Tang, Melody Wen, Gerhard Koenig, Jean-François Blain, Duane A Burnett
Herein we describe the design, synthesis, and evaluation of a novel series of oxadiazine-based gamma secretase modulators obtained via isosteric amide replacement and critical consideration of conformational restriction. Oxadiazine lead 47 possesses good in vitro potency with excellent predicted CNS drug-like properties and desirable ADME/PK profile. This lead compound demonstrated robust Aβ42 reductions and subsequent Aβ37 increases in both rodent brain and CSF at 30 mg/kg dosed orally.
March 23, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/25501811/the-dynamic-conformational-landscape-of-gamma-secretase
#2
Nadav Elad, Bart De Strooper, Sam Lismont, Wim Hagen, Sarah Veugelen, Muriel Arimon, Katrien Horré, Oksana Berezovska, Carsten Sachse, Lucía Chávez-Gutiérrez
The structure and function of the gamma-secretase proteases are of great interest because of their crucial roles in cellular and disease processes. We established a novel purification protocol for the gamma-secretase complex that involves a conformation- and complex-specific nanobody, yielding highly pure and active enzyme. Using single particle electron microscopy, we analyzed the gamma-secretase structure and its conformational variability. Under steady-state conditions, the complex adopts three major conformations, which differ in overall compactness and relative position of the nicastrin ectodomain...
February 1, 2015: Journal of Cell Science
https://www.readbyqxmd.com/read/27908834/gamma-secretase-inhibitor-impairs-epithelial-to-mesenchymal-transition-induced-by-tgf-%C3%AE-in-ovarian-tumor-cell-lines
#3
M C Pazos, D Abramovich, A Bechis, P Accialini, F Parborell, M Tesone, G Irusta
Ovarian cancer is characterized by being highly metastatic, a feature that represents the main cause of failure of the treatment. This study investigated the effects of γ-secretase inhibition on the TGF-β-induced epithelial-mesenchymal transition (EMT) process in ovarian cancer cell lines. SKOV3 cells incubated in the presence of TGF-β showed morphological and biochemical changes related to EMT, which were blocked by co-stimulation with TGF-β and the γ-secretase inhibitor DAPT. In SKOV3 and IGROV1 cells, the co-stimulation blocked the cadherin switch and the increase in the transcription factors Snail, Slug, Twist and Zeb1 induced by TGF-β...
January 15, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28186678/gamma-secretase-inhibitor-ix-gsi-impairs-concomitant-activation-of-notch-and-wnt-beta-catenin-pathways-in-cd44-gastric-cancer-stem-cells
#4
Samarpita Barat, Xi Chen, Khac Cuong Bui, Przemyslaw Bozko, Julian Götze, Matthias Christgen, Till Krech, Nisar P Malek, Ruben R Plentz
Cancer stem cells (CSC) are associated with tumor resistance and are characterized in gastric cancer (GC). Studies have indicated that Notch and wnt-beta-catenin pathways are crucial for CSC development. Using CD44(+) CSCs, we investigated the role of these pathways in GC carcinogenesis. We performed cell proliferation, wound healing, invasion, tumorsphere, and apoptosis assays. Immunoblot analysis of downstream signaling targets of Notch and wnt-beta-catenin were tested after gamma-secretase inhibitor IX (GSI) treatment...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28278367/a-systematic-review-and-critical-evaluation-of-reported-pathogenic-sequence-variants-in-hidradenitis-suppurativa
#5
REVIEW
J W Frew, D A Vekic, J Woods, G D Cains
Hidradenitis suppurativa (HS) is a severe chronic inflammatory disorder characterized by recurrent painful deep-seated nodules with a predilection to the apocrine-bearing areas of skin. A minority of cases of HS are due to mutations in the γ-secretase complex. Contention exists surrounding the pathogenicity of sequence variants and their effects upon Notch signalling. This systematic review was registered with PROSPERO (CRD42016041425) and was conducted in line with the PRISMA statement. Eligibility criteria for this review included published case reports, case series and reviews that identified sequence variants or protein or functional studies from patients with HS...
October 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28460541/why-do-trials-for-alzheimer-s-disease-drugs-keep-failing-a-discontinued-drug-perspective-for-2010-2015
#6
Dev Mehta, Robert Jackson, Gaurav Paul, Jiong Shi, Marwan Sabbagh
There are dozens of drugs in development for AD with billions of dollars invested. Despite the massive investment in AD drugs and a burgeoning pipeline, there have been more setbacks and failures than treatment successes. Areas covered: The classes of drugs that have failed to date include the monoclonal antibodies, the gamma secretase inhibitors, dimebon, neurochemical enhancers, and one tau drug. Data for these compounds were sought through a PubMed search and a clinicaltrials.gov search. Expert opinion: The obvious question to be posed is: Why are they failing? Is the treatment of symptomatic dementia too late? Are the therapeutic targets incorrect? Are the clinical methodologies imprecise, misleading, or inaccurate? This review summarizes the drugs that have failed during 2010-2015 and offers possible theories as to why they have failed...
June 2017: Expert Opinion on Investigational Drugs
https://www.readbyqxmd.com/read/27007185/gamma-secretase-modulators-new-alzheimer-s-drugs-on-the-horizon
#7
REVIEW
Matthew G Bursavich, Bryce A Harrison, Jean-François Blain
The rapidly aging population desperately requires new therapies for Alzheimer's disease. Despite years of pharmaceutical research, limited clinical success has been realized, with several failed disease modification therapies in recent years. On the basis of compelling genetic evidence, the pharmaceutical industry has put a large emphasis on brain beta amyloid (Aβ) either through its removal via antibodies or by targeting the proteases responsible for its production. In this Perspective, we focus on the development of small molecules that improve the activity of one such protease, gamma secretase, through an allosteric binding site to preferentially increase the concentration of the shorter non-amyloidogenic Aβ species...
August 25, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28320827/structural-and-chemical-biology-of-presenilin-complexes
#8
Douglas S Johnson, Yue-Ming Li, Martin Pettersson, Peter H St George-Hyslop
The presenilin proteins are the catalytic subunits of a tetrameric complex containing presenilin 1 or 2, anterior pharynx defective 1 (APH1), nicastrin, and PEN-2. Other components such as TMP21 may exist in a subset of specialized complexes. The presenilin complex is the founding member of a unique class of aspartyl proteases that catalyze the γ, ɛ, ζ site cleavage of the transmembrane domains of Type I membrane proteins including amyloid precursor protein (APP) and Notch. Here, we detail the structural and chemical biology of this unusual enzyme...
March 20, 2017: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/27133790/physiological-and-pathological-roles-of-the-%C3%AE-secretase-complex
#9
REVIEW
Courtney M Carroll, Yue-Ming Li
Gamma-secretase (GS) is an enzyme complex that cleaves numerous substrates, and it is best known for cleaving amyloid precursor protein (APP) to form amyloid-beta (Aβ) peptides. Aberrant cleavage of APP can lead to Alzheimer's disease, so much research has been done to better understand GS structure and function in hopes of developing therapeutics for Alzheimer's. Therefore, most of the attention in this field has been focused on developing modulators that reduce pathogenic forms of Aβ while leaving Notch and other GS substrates intact, but GS provides multiple avenues of modulation that could improve AD pathology...
September 2016: Brain Research Bulletin
https://www.readbyqxmd.com/read/27192874/-functional-properties-of-gamma-secretase-in-the-yeast-reconstitution-system
#10
REVIEW
Eugene Futai
No abstract text is available yet for this article.
April 2016: Seikagaku. the Journal of Japanese Biochemical Society
https://www.readbyqxmd.com/read/26322584/%C3%AE-secretase-processing-of-app-inhibits-neuronal-activity-in-the-hippocampus
#11
Michael Willem, Sabina Tahirovic, Marc Aurel Busche, Saak V Ovsepian, Magda Chafai, Scherazad Kootar, Daniel Hornburg, Lewis D B Evans, Steven Moore, Anna Daria, Heike Hampel, Veronika Müller, Camilla Giudici, Brigitte Nuscher, Andrea Wenninger-Weinzierl, Elisabeth Kremmer, Michael T Heneka, Dietmar R Thal, Vilmantas Giedraitis, Lars Lannfelt, Ulrike Müller, Frederick J Livesey, Felix Meissner, Jochen Herms, Arthur Konnerth, Hélène Marie, Christian Haass
Alzheimer disease (AD) is characterized by the accumulation of amyloid plaques, which are predominantly composed of amyloid-β peptide. Two principal physiological pathways either prevent or promote amyloid-β generation from its precursor, β-amyloid precursor protein (APP), in a competitive manner. Although APP processing has been studied in great detail, unknown proteolytic events seem to hinder stoichiometric analyses of APP metabolism in vivo. Here we describe a new physiological APP processing pathway, which generates proteolytic fragments capable of inhibiting neuronal activity within the hippocampus...
October 15, 2015: Nature
https://www.readbyqxmd.com/read/27390881/membrane-trafficking-and-proteolytic-activity-of-%C3%AE-secretase-in-alzheimer-s-disease
#12
REVIEW
Kunihiko Kanatsu, Taisuke Tomita
γ-Secretase is an intramembrane-cleaving protease that generates various forms of amyloid-β peptides (Aβ) that accumulate in the brains of Alzheimer's disease (AD) patients. The intracellular trafficking and subcellular localization of γ-secretase are linked to both qualitative and quantitative changes in Aβ production. However, the precise intracellular localization of γ-secretase as well as its detailed regulatory mechanisms have remained elusive. Recent genetic studies on AD provide ample evidence that alteration of the subcellular localization of γ-secretase contributes to the pathogenesis of AD...
September 1, 2016: Biological Chemistry
https://www.readbyqxmd.com/read/10801777/inhibiting-amyloid-precursor-protein-c-terminal-cleavage-promotes-an-interaction-with-presenilin-1
#13
G Verdile, R N Martins, M Duthie, E Holmes, P H St George-Hyslop, P E Fraser
Presenilin 1 (PS1) plays a pivotal role in the production of the amyloid-beta protein, which is central to the pathogenesis of Alzheimer's disease. It has been demonstrated that PS1 regulates the gamma-secretase proteolysis of the amyloid precursor protein (APP) C-terminal fragment (APP-C100), which is the final step in amyloid-beta protein production. The mechanism and detailed pathway of this PS1 activity has yet to be fully resolved, but it may be due to a presenilin-controlled trafficking of the APP fragment or possibly an inherent PS1 proteolytic activity...
July 7, 2000: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26075716/sodium-chloride-increases-a%C3%AE-levels-by-suppressing-a%C3%AE-clearance-in-cultured-cells
#14
Xiao-Juan Cheng, Yuan Gao, Yu-Wu Zhao, Xiao-Dong Cheng
Recent studies suggest that high-salt diet is associated with cognitive decline in human and mouse. The fact that genetic factors account for less than 50% cases of sporadic Alzheimer's disease (AD) highlights the important contribution of environmental factors, such as high-salt diet, in AD pathogenesis. However, whether and how high-salt diet fits the "amyloid cascade" hypothesis remains unexplored. Here, we show sodium chloride (NaCl) could increase Aβ levels in the medium of HEK293 cells overexpressing amyloid precursor protein (APP) or C99 fragment...
2015: PloS One
https://www.readbyqxmd.com/read/26059427/production-of-active-glycosylation-deficient-%C3%AE-secretase-complex-for-crystallization-studies
#15
Andrés Ricardo López, Mitko Dimitrov, Hermeto Gerber, Virginie Braman, David L Hacker, Florian M Wurm, Patrick C Fraering
Alzheimer's disease (AD)-associated γ-secretase is a ubiquitously expressed multi-subunit protease complex embedded in the lipid bilayer of cellular compartments including endosomes and the plasma membrane. Although γ-secretase is of crucial interest for AD drug discovery, its atomic structure remains unresolved. γ-Secretase assembly and maturation is a multistep process, which includes extensive glycosylation on nicastrin (NCT), the only γ-secretase subunit having a large extracellular domain. These posttranslational modifications lead to protein heterogeneity that likely prevents the three-dimensional (3D) crystallization of the protease complex...
December 2015: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/25996556/akt1-phosphorylates-nicastrin-to-regulate-its-protein-stability-and-activity
#16
Eun-Hye Jo, Ji-Seon Ahn, Jung-Soon Mo, Ji-Hye Yoon, Eun-Jung Ann, Hyeong-Jin Baek, Hye-Jin Lee, Seol-Hee Kim, Mi-Yeon Kim, Hee-Sae Park
The gamma-secretase is a multiprotein complex that cleaves many type-I membrane proteins, such as the Notch receptor and the amyloid precursor protein. Nicastrin (NCT) is an essential component of the multimeric gamma-secretase complex and functions as a receptor for gamma-secretase substrates. In this study, we found that Akt1 markedly regulated the protein stability of NCT. Importantly, the kinase activity of Akt1 was essential for the inhibition of gamma-secretase activity through degradation of NCT. Notably, the protein level of endogenous NCT was higher in shAkt1-expressing cells than in shCon-expressing cells...
September 2015: Journal of Neurochemistry
https://www.readbyqxmd.com/read/18266764/active-gamma-secretase-is-localized-to-detergent-resistant-membranes-in-human-brain
#17
Ji-Yeun Hur, Hedvig Welander, Homira Behbahani, Mikio Aoki, Jenny Frånberg, Bengt Winblad, Susanne Frykman, Lars O Tjernberg
Several lines of evidence suggest that polymerization of the amyloid beta-peptide (Abeta) into amyloid plaques is a pathogenic event in Alzheimer's disease (AD). Abeta is produced from the amyloid precursor protein as the result of sequential proteolytic cleavages by beta-secretase and gamma-secretase, and it has been suggested that these enzymes could be targets for treatment of AD. gamma-Secretase is an aspartyl protease complex, containing at least four transmembrane proteins. Studies in cell lines have shown that gamma-secretase is partially localized to lipid rafts, which are detergent-resistant membrane microdomains enriched in cholesterol and sphingolipids...
March 2008: FEBS Journal
https://www.readbyqxmd.com/read/25523933/structural-biology-of-presenilin-1-complexes
#18
REVIEW
Yi Li, Christopher Bohm, Roger Dodd, Fusheng Chen, Seema Qamar, Gerold Schmitt-Ulms, Paul E Fraser, Peter H St George-Hyslop
The presenilin genes were first identified as the site of missense mutations causing early onset autosomal dominant familial Alzheimer's disease. Subsequent work has shown that the presenilin proteins are the catalytic subunits of a hetero-tetrameric complex containing APH1, nicastrin and PEN-2. This complex (variously termed presenilin complex or gamma-secretase complex) performs an unusual type of proteolysis in which the transmembrane domains of Type I proteins are cleaved within the hydrophobic compartment of the membrane...
December 18, 2014: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/25052851/gamma-secretase-activating-protein-is-a-substrate-for-caspase-3-implications-for-alzheimer-s-disease
#19
Jin Chu, Jian-Guo Li, Yash B Joshi, Phillip F Giannopoulos, Nicholas E Hoffman, Muniswamy Madesh, Domenico Praticò
BACKGROUND: A major feature of Alzheimer's disease (AD) is the accumulation of amyloid-beta (Aβ), whose formation is regulated by the gamma-secretase complex and its activating protein (also known as GSAP). Because GSAP interacts with gamma-secretase without affecting the cleavage of Notch, it is an ideal target for a viable anti-Aβ therapy. However, despite much interest in this protein, the mechanisms involved in its neurobiology are unknown. METHODS: Postmortem brain tissue samples from AD patients, transgenic mouse models of AD, and neuronal cells were used to investigate the molecular mechanism involved in GSAP formation and subsequent amyloidogenesis...
April 15, 2015: Biological Psychiatry
https://www.readbyqxmd.com/read/23265086/the-backbone-dynamics-of-the-amyloid-precursor-protein-transmembrane-helix-provides-a-rationale-for-the-sequential-cleavage-mechanism-of-%C3%AE-secretase
#20
Oxana Pester, Paul J Barrett, Daniel Hornburg, Philipp Hornburg, Rasmus Pröbstle, Simon Widmaier, Christoph Kutzner, Milena Dürrbaum, Aphrodite Kapurniotu, Charles R Sanders, Christina Scharnagl, Dieter Langosch
The etiology of Alzheimer's disease depends on the relative abundance of different amyloid-β (Aβ) peptide species. These peptides are produced by sequential proteolytic cleavage within the transmembrane helix of the 99 residue C-terminal fragment of the amyloid precursor protein (C99) by the intramembrane protease γ-secretase. Intramembrane proteolysis is thought to require local unfolding of the substrate helix, which has been proposed to be cleaved as a homodimer. Here, we investigated the backbone dynamics of the substrate helix...
January 30, 2013: Journal of the American Chemical Society
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