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Lactate production in E. coli

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Hyun Ju Kim, Bo Kyeng Hou, Sung Gun Lee, Joong Su Kim, Dong-Woo Lee, Sang Jun Lee
Most current metabolic engineering applications rely on the inactivation of unwanted reactions and the amplification of product-oriented reactions. All of the biochemical reactions involved with cellular metabolism are tightly coordinated with the electron flow, which depends on the cellular energy status. Thus, the cellular metabolic flux can be controlled either by modulation of the electron flow or the regulation of redox reactions. This study analyzed the genome-wide anaerobic fermentation products of 472 Escherichia coli single gene knockouts, which comprised mainly of dehydrogenases, oxidoreductases, and redox-related proteins...
July 2013: Metabolic Engineering
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