We have located links that may give you full text access.
JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
Indirect sandwich enzyme-linked immunosorbent assay for rapid detection of Streptococcus pneumoniae type 3 antigen.
Journal of Clinical Microbiology 1980 June
An indirect sandwich enzyme-linked immunosorbent assay for the detection of the polysaccharide antigen of type 3 pneumococcus (SSS-III) is reported. Polystyrene balls with attached anti-SSS-III antibody serve as the solid phase, and antigen is detected using an alkaline phosphatase-labeled antiglobulin conjugate. The reaction is quantitated by spectrophotometry. Concentrations of antigen are estimated by comparison with standard curves prepared with purified SSS-III. For this assay, the practical lower limit of detection of SSS-III is approximately 2 to 3 ng/ml, thus making the test sensitivity about 25 times that reported for counterimmunoelectrophoresis. In preliminary tests with simulated human clinical specimens, none of the body fluids tested (spinal fluid, serum, urine, and sputum) interfered with detection of antigen, nor did they produce false-positive or false-negative results. Streptococcus pneumoniae type 3 whole organisms were readily detected in simulated clinically positive sputum specimens. Cross-reactions were not observed with Haemophilus influenzae type b, group B Streptococcus, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
Full text links
Trending Papers
A Personalized Approach to the Management of Congestion in Acute Heart Failure.Heart International 2023
Potential Mechanisms of the Protective Effects of the Cardiometabolic Drugs Type-2 Sodium-Glucose Transporter Inhibitors and Glucagon-like Peptide-1 Receptor Agonists in Heart Failure.International Journal of Molecular Sciences 2024 Februrary 21
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app