[Detection of some markers of the early phase of the inflammatory and reparative process in mechanical skin injuries].

OBJECTIVE: To examine the applicability of IHC staining method: with TGF-β1 antibodies (serial examination, statistically processed results) and with mast cell tryptase antibodies for injuries vitality determination.

MATERIAL AND METHODS: 261 skin autopsy samples with mechanical injuries from 29 persons were divided to 3 groups (87 in each group): vital injuries, postmortal injuries, control non-injured samples. A routine histological examination using standard H&E stain and IHC both with TGF-β1 and mast cells tryptase antibodies was performed.

RESULTS AND CONCLUSION: The positive TGF-β1 staining (score 2-3) was found in keratinocytes in vitally injured skin and the negative or weak one (score 0-1) was found in control postmortally injured and non-injured samples. Additionally, dermal TGF-β1 expression was found in some vitally injured skin samples. The difference between vitally injured skin and control samples was statistically significant ( p <0.05). No significant difference of dermal mast cells density in groups 1, 2, 3 was found.