We have located links that may give you full text access.
Further characterization and standardization of mouse monoclonal antibodies reacting with M/N blood group antigens.
The M/N-blood-group system is very suitable for investigating the reactivity of monoclonal antibodies because the antigenic structure seems to be very dependent on influences of the microenvironment, whereas on the other hand the biochemistry of these antigens is very well known. The distribution of these antigens within the population makes this system very interesting for forensic investigations and therefore one needs specific reagents with constant quality. We have immunized BALB/c mice with whole red blood cells and obtained a series of monoclonal antibodies. Some of these reacted with epitopes which are very dependent on pH, temperature and buffers concerning the binding of the antibodies and the specificity. By standardizing the monoclonal preparation in this respect it was possible to obtain specific reagents. In addition we found antibodies reacting with epitopes which were not influenced by the microenvironment. These antibodies always gave specific results. To get further criteria for standardization of these reagents we have adapted the method of flow cytometry for binding studies of monoclonal antibodies against red blood cell antigens.
Full text links
Related Resources
Trending Papers
Heart failure with preserved ejection fraction: diagnosis, risk assessment, and treatment.Clinical Research in Cardiology : Official Journal of the German Cardiac Society 2024 April 12
Proximal versus distal diuretics in congestive heart failure.Nephrology, Dialysis, Transplantation 2024 Februrary 30
World Health Organization and International Consensus Classification of eosinophilic disorders: 2024 update on diagnosis, risk stratification, and management.American Journal of Hematology 2024 March 30
Efficacy and safety of pharmacotherapy in chronic insomnia: A review of clinical guidelines and case reports.Mental Health Clinician 2023 October
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app