Klebsiella indica sp. nov., isolated from the surface of a tomato.

A novel bacterial strain, designated TOUT106T , was isolated from the surface of a tomato. The cells were rod-shaped, Gram-negative, encapsulated and non-motile. Strain TOUT106T grows best at 28 °C and pH 7.0 and can tolerate up to 2 % (w/v) NaCl. Based on 16S rRNA gene phylogeny, strain TOUT106T was placed close to the Salmonella clade, with close similarity to Salmonella enterica subsp. arizonae strain NCTC 8297T (98.42 %). Results of genome-based phylogenetic analysis revealed that strain TOUT106T is placed well in the Klebsiella-Raoultella clade, by forming a distinct branch with Klebsiella michiganensis DSM25444T , Klebsiella oxytoca NCTC132727T , Klebsiella grimontii 06D021T and Klebsiella pasteurii SB6412T . The genomic DNA G+C content of strain TOUT106T is 53.53 mol%. The average nucleotide identity values of TOUT106T were less than 86.5 % with closely related members of the family Enterobacteriaceae . The major fatty acids of strain TOUT106T were C16 : 0 , C17:0 cyclo, C14:0 3OH/C16:1 iso, C14 : 0 , C19:0 cyclo ω8 c , C18:1 ω6 c /C18:1 ω7 c , C12 : 0 and C16:1 ω7 c /C16:1 ω6 c . Strain TOUT106T showed differences in physiological, phenotypic and protein profiles by MALDI-TOF MS compared to its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain TOUT106T could be distinguished from the recognized species of the genus Klebsiella . It is suggested to represent a novel species of this genus, for which the name Klebsiella indica sp. nov. is proposed. The type strain is TOUT106T (=MCC 2901T =KACC 21384T =JCM 33718T ).