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Genome Mining and Enzymatic Total Biosynthesis of Purincyclamide.
Organic Letters 2019 August 14
A cyclodipeptide synthase-containing gene cluster ( pcm ) was identified in Streptomyces chrestomyceticus NA4264 through genome mining. Heterologous expression of the cryptic pcm gene cluster in Streptomyces albus led to the production of a new highly modified cyclodipeptide named purincyclamide ( 1 ). Bioinformatic analysis and gene knockout experiments revealed the biosynthetic pathway. The production of 1 was achieved through a one-pot enzymatic reaction.
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