Three-dimensional Spherical Gelatin Bubble-based Scaffold Improves the Myotube Formation of H9c2 Myoblasts.

Microenvironmental factors including physical and chemical cues can regulate stem cells as well as terminally differentiated cells to modulate their biological function and differentiation. However, one of the physical cues, the substrate's dimensionality , has not studied extensively. In this study, the flow-focusing method with a microfluidic device was used to generate gelatin bubbles to fabricate highly ordered three-dimensional (3D) scaffolds. Rat H9c2 myoblasts were seeded into the 3D gelatin bubble-based scaffolds and compared to those grown on 2D gelatin-coating substrates to demonstrate the influences of spatial cues on cell behaviours. Relative to cells on the 2D substrates, the H9c2 myoblasts were featured by a good survival, normal mitochondrial activity, but slower cell proliferation within the 3D scaffolds. The cortical actin filaments of H9c2 cells were localized close to the cell membrane when cultured on the 2D substrates, while the F-actins distributed uniformly and occupied most of the cell cytoplasm within the 3D scaffolds. H9c2 myoblasts fused as multinuclear myotubes within the 3D scaffolds without any induction, but cells cultured on the 2D substrates had a relatively lower fusion index even differentiation medium was provided. Although these was no difference in actin alpha 1 and myosin heavy chain 1, H9c2 cells had a higher myogenin mRNA level in the 3D scaffolds than those of on the 2D substrates. This study reveals that the dimensionality influences differentiation and fusion of myoblasts. This article is protected by copyright. All rights reserved.