Spermatophore producing process and sperm transfer in Phytoseiulus persimilis.

In phytoseiid mites, the spermatophore is an intermediate, isolated structure where sperms are stored during mating. In the present study, the producing process of Phytoseiulus persimilis spermatophore is described in details. Its shape, and the number and shape of sperms inside, are also analyzed, each as affected by coupling time. Based on our results, the spermatophore of P. persimilis is pyriform, and is produced from the male genital opening within 3 min since mating started. When produced, the spermatophore is transferred along the capitular groove to the cheliceral base, where it is adhered to one of the two small holes at the bases of the chelicerae. Seminal fluid passes through the hole, the hollow spermatodactyl, the opening on the spermatodactyl tip, and enters the insemination pore to reach the spermatheca of the female. No sperm was observed in spermatophores obtained 5 min after mating started. The number of sperms increased in the next 10 min, reached its maximum (ca. 52 sperms per spermatophore), and then decreased. All sperms were released in 75 min after mating started. Sperms are slender in shape and on average 3.9 ± 0.3 µm long.