Aptamer-modified magnetic metal-organic framework MIL-101 for highly efficient and selective enrichment of ochratoxin A.

A facile and efficient strategy is developed to modify aptamers on the surface of the magnetic metal-organic framework MIL-101 for the rapid magnetic solid-phase extraction of ochratoxin A. To the best of our knowledge, this is the first attempt to create a robust aptamer-modified magnetic MIL-101 with covalent bonding for the magnetic separation and enrichment of ochratoxin A. The saturated adsorption of ochratoxin A by aptamer-modified magnetic MIL-101 was 7.9 times greater than that by magnetic metal-organic framework MIL-101 due to the former's high selective recognition as well as good stability. It could be used for extraction more than 12 times with no significant changes in the extraction efficiency. An aptamer-modified magnetic MIL-101-based method of magnetic solid-phase extraction combined with ultra high performance liquid chromatography with tandem mass spectrometry was developed for the determination of trace ochratoxin A with limit of detection of 0.067 ng/L. Ochratoxin A of 4.53-13.7 ng/kg was determined in corn and peanut samples. The recoveries were in the range 82.8-108% with a relative standard deviation (n = 5) of 4.5-6.5%. These results show that aptamer-modified magnetic MIL-101 exhibits selective and effective enrichment performance and have excellent potential for the analysis of ultra-trace targets from complex matrices.