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Simultaneous quantification of oxybutynin and its active metabolite N-desethyl oxybutynin in rat plasma by ultra high performance liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study of oxybutynin transdermal patch.
Biomedical Chromatography : BMC 2018 December 8
A rapid, selective and sensitive ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously determine oxybutynin (OB) and its active metabolite N-desethyl oxybutynin (DEOB) in rat plasma. Sample of 0.1 mL plasma was extracted with n-hexane. Chromatographic separation was performed on a UPLC BEH C18 column(2.1 × 100 mm I.D.,1.7 μm) with mobile phase of methanol-water (containing 2 mmol/L ammonium acetate and 0.1% formic acid) (90:10, v/v). The detection was performed in positive selected reaction monitoring (SRM) mode. Each plasma sample was chromatographed within 3 min. The linear calibration curves were obtained in the concentration range of 0.0944-189 ng/mL (r ≥0.99) for OB and 0.226-18.0 ng/mL (r ≥0.99) for DEOB. The intra- and inter-day precision (relative standard deviation, R.S.D.) values were not more than 14% and the accuracy (relative error, R.E.) was within ±7.6%. The method herein described was superior to previous methods in quantitation oxybutynin with three product ions and successfully applied to pharmacokinetic study of oxybutynin and its active metabolite N-desethyl oxybutynin in rat plasma after transdermal administration.
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