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Combinatorial Peptide Array Synthesis Based on Microfluidic Impact Printing.
ACS Combinatorial Science 2018 December 7
In this paper, a novel inkjet printing technique, micro impact printing (MI printing), is applied for the first time to combinatorial peptide array synthesis on amine functionalized micro disc arrays through standard Fmoc chemistry. MI printing shows great advantages in combinatorial peptide array synthesis compared with other printing techniques, including 1) a disposable cartridge; 2) a small spot size (80 μm) increases array density; 3) minimal loading volume (0.6 μL) and dead volume (<0.1 μL) reduce chemical waste; and 4) multiplexability for up to 5 channels. Using this synthesis approach, a tetrapeptide library with 625 permutations was constructed and then applied for the screening of ligands targeting α4β1 integrin on Jurkat cells.
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