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Reversible S-glutathionylation of human 6-pyruvoyl tetrahydropterin synthase protects its enzymatic activity.
Journal of Biological Chemistry 2018 December 5
6-Pyruvoyl tetrahydropterin synthase (PTS) converts 7, 8-dihydroneopterin triphosphate into 6-pyruvoyltetrahydropterin and is a critical enzyme for the de novo synthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases. Neopterin derived from 7,8-dihydroneopterin triphosphate is secreted by monocytes/macrophages, and is a well-known biomarker for cellular immunity. Because PTS activity in the cell can be a determinant of neopterin production, here we used recombinant human PTS protein to investigate how its activity is regulated, especially depending on redox conditions. Human PTS has two cysteines: Cys-43 at the catalytic site and Cys-10 at the N-terminus. PTS can be oxidized and consequently inactivated by H2 O2 treatment, oxidized glutathione, or S -nitrosoglutathione, and determining the oxidized modifications of PTS induced by each oxidant by MALDI-TOF MS, we show that PTS is S -glutathionylated in the presence of glutathione and H2 O2 S -Glutathionylation at Cys-43 protected PTS from H2 O2 -induced irreversible sulfinylation and sulfonylation. We also found that PTS expressed in HeLa and THP-1 cells is reversibly modified under oxidative stress conditions. Our findings suggest that PTS activity and S -glutathionylation is regulated by the cellular redox environment and that reversible S -glutathionylation protects PTS against oxidative stress.
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