Meropenem and ciprofloxacin in complicated gastric surgery for cancer patients: A simple SPE-UHPLC-PDA method for their determination in human plasma.

A simple and rapid ultra high-performance liquid chromatographic (UHPLC) for the simultaneous determination of meropenem and ciprofloxacin in human plasma was developed and validate. All the analytes were separated in less than 5 min. A solid-phase extraction method was applied from samples preparation. Analytical separation was performed on a Poroshell SB C18 column (50 x 2.1 mm, 2.7 μm particle size) with PDA detection. Meropenem and ciprofloxacin were carried out at a wavelength 300 and 277 nm, respectively. The mobile phase was a mixture of acetonitrile -10mM ammonium acetate - methanol in gradient elution. The method has been validated for both drugs in gastric surgery for cancer patients. The method showed good linearity with correlation coefficients, r2 = 0.994 for the two drugs, as well as high precision (RSD% < 10.5% in each case), accuracy ranged from -5.8% to +6.0 %. The limit of quantitation of the two drugs was established at 0.02 and 0.01 μg/mL, respectively. Meropenem, ciprofloxacin and the internal standard were extracted from human plasma with a mean recovery ranging from 92.5 to 98.6%. The method was applied to quantify the drugs dosage in complicated gastric surgery patients.