Fast response fluorogenic probe for visualizing hypochlorite in living cells and Zebrafishes.

A novel fast response and visible fluorogenic probe (D1) for monitoring hypochlorite (ClO-) has been developed based on specific ClO- removal of a C=N bond. The response of the probe to ClO- showed increases linearly, and the fluorescence intensity heighten about 25 times. D1 responses to ClO- were observed with high selectivity and sensitivity within 10 s by naked eyes. D1 can not only detect the level of hypochlorite in vitro such as urine, but also monitoring hypochlorite content in extremely cold condition as low as -78 oC. Meanwhile, the good biocompatibility guaranteed the use of D1 to detect intracellular ClO- under confocal microscopy. Moreover, D1 has been successfully applied to monitor the exogenous and endogenous ClO- in Zebrafishes with fluorescence imaging.