Development of Synchrotron Footprinting at NSLS and NSLS-II.

X-ray synchrotron footprinting is a rapidly growing technique ideal for the analysis of solution-state structure and dynamics of macromolecules. First developed in the 1990's at the National Synchrotron Light Source, instrumentation to expand the technology has continuously pushed the boundaries of biological systems that can be studied using the technique. Dedicated synchrotron beamlines, first X28C at NSLS, then XFP (17-BM) at NSLS-II provide an important resource for examining fundamental biological mechanisms of folding, ligand binding, catalysis, splicing, transcription, translation, and macromolecular assembly.