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Wet NH 3 -Triggered NH 2 -MIL-125(Ti) Structural Switch for Visible Fluorescence Immunoassay Impregnated on Paper.
Analytical Chemistry 2018 December 19
This work has looked to explore an innovative and powerful visible fluorescence immunoassay method through wet NH3 -triggered structural change of NH2 -MIL-125(Ti) impregnated on paper for the detection of carcinoembryonic antigen (CEA). Gold nanoparticles heavily functionalized with glutamate dehydrogenase (GDH) and secondary antibody were used for generation of wet NH3 with a sandwiched immunoassay format. Paper-based analytical device (PAD) coated with NH2 -MIL-125(Ti) exhibited good visible fluorescence intensity through wet NH3 -triggeried structural change with high accuracy and reproducibility. Moreover, NH2 -MIL-125(Ti)-based PAD displayed two visual modes of fluorescence color and physical color with the naked eye and allowed the detection of CEA at a concentration as low as 0.041 ng mL-1 . Importantly, the PAD-based assay provides promise for use in the mass production of miniaturized devices and opens new opportunities for protein diagnostics and biosecurity.
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