Curcumin loading potentiates the neuroprotective efficacy of Fe 3 O 4 magnetic nanoparticles in cerebellum cells of schizophrenic rats.

BACKGROUND: The aim of this study was to investigate the neurotoxic effects of Fe3 O4 magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model.

METHODS: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (Fe3 O4 magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of Fe3 O4 magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove Fe3 O4 magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation, ATP level, cytochrome c release and histopathology of cerebellums were determined in brains of schizophrenic rats.

RESULTS: We showed that effective treatment with CMN reduced or prevented Fe3 O4 magnetic-induced oxidative stress and mitochondrial dysfunction in the rat brain probably, as well as mitochondrial complex II activity, MMP, and ATP level were remarkably reduced in the cerebellum mitochondria of treated group toward control (p < 0.05). Therewith, ROS generation, and cytochrome c release were notably (p < 0.05) increased in the cerebellum mitochondria of treated group compared with control group.

CONCLUSION: Taken together, Fe3 O4 magnetic- CurNPs exhibits potent antineurotoxicity activity in cerebellums of schizophrenic rats. This approach can be extended to preclinical and clinical use and may have importance in schizophernia treatment in the future. To our knowledge this is the first report that provides the Fe3 O4 magnetic- CurNPs could enhance the neuroprotective effects of CMN in the Schizophrenia.