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An Electrochemical Aptasensor Based on Sulfur-Nitrogen Co-Doped Ordered Mesoporous Carbon and Thymine-Hg2+-Thymine Mismatch Structure for Hg2+ Detection.
ACS Sensors 2018 November 10
A renewable electrochemical aptasensor was proposed for super sensitive determination of Hg2+. The novel aptasensor, based on sulfur-nitrogen co-doped ordered mesoporous carbon (SN-OMC) and thymine-Hg2+-thymine (T-Hg2+-T) mismatch structure, used ferrocene as signal molecules to achieve the conversion of current signals. In the absence of Hg2+, the thiol modified T-rich probe 1 spontaneously formed a hairpin structure by base pairing. After hybridizing with the ferrocene-labeled probe 2 in the presence of Hg2+, the hairpin structure of probe 1 was opened due to the preferentially formation of T-Hg2+-T mismatch structure and the ferrocene signal molecules approached the modified electrode surface. SN-OMC with high specific surface area and ample active sites acted as a signal amplification element in electrochemical sensing. The sensitive determination of Hg2+ can be actualized by analyzing the relationship between the change of oxidation current caused by ferrocene signal molecules and the Hg2+ concentrations. The aptasensor had a fine linear correlation in the range of 0.001 - 1000 nM with detection limit of 0.45 pM. The aptasensor also displayed a good response in real sample detection and provided a promising possibility for in-situ detection.
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