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Does Fluid Temperature Affect Corneal Endothelium-Descemet Membrane Scroll Formation? An In Vitro Study.

Objectives: To investigate whether unfolding time of Descemet membrane (DM) graft rolls changes at various fluid temperatures.

Materials and Methods: The study was prospective, ex vivo , and experimental. The study was conducted at the tertiary center for corneal disease in Adana Numune Training and Research Hospital between June 2014 and June 2015. DMs were divided into 4 categories according to baseline roll tightness and these were distributed among 4 different groups using 4 different balanced salt solution (BSS) temperatures (8, 16, 23, and 36 °C). Sixteen donor corneas were obtained from the hospital eye bank.

Results: DM roll formations may vary according to the donor cornea received. Some form tighter rolls while others can form a more open roll. No differences in roll tightness were observed in any of the DM rolls after 5 or 10 minutes in the different BBS temperatures. In all groups, neither tightening nor opening was observed in DM roll formations.

Conclusion: Different BSS temperatures were found to have no effects on DM unfolding time in this study.

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