Coordinated regulation of intracellular fascin distribution governs tumor microvesicle release and invasive cell capacity.

Tumor cell invasion is one result of the bidirectional interactions occurring between tumor cells and the surrounding milieu. The ability of tumor cells to invade through the extracellular matrix is in part regulated by the formation of a class of protease-loaded extracellular vesicles, called tumor microvesicles (TMVs), which are released directly from the cell surface. Here we show that the actin bundling protein, fascin, redistributes to the cell periphery in a ternary complex with podocalyxin and ezrin, where it promotes TMV release. The peripheral localization of fascin is prompted by the loss of Rab35 signaling which in turn unleashes ARF6 activation. The results, is a mechanism through which Rab35 and ARF6 cooperatively and simultaneously regulate the distribution and localization of fascin; and promote oncogenic signaling which leads to TMV release while inhibiting invadopodia formation. These studies are clinically significant as fascin-loaded TMVs can be detected in bodily fluids and elevated fascin expression coupled with low Rab35 levels correlates with poor overall survival in some cancers.