Differential Gene Expression of RNA-Seq Experiments of Primo Vessel in Rabbit Lymph.

For the connectome of primo vascular system some long-type primo vessels dyed with Alcian blue injected into inguinal nodes, abdominal node, and axially nodes were visualized, which passed over around the vena cava of rabbit. The Alcian blue dye revealed primo vessels and colored blue in the rabbit lymph vessels. The length of a long-type primo vessels was an 18 cm average, of which diameters were about 20∼30 μm, and the lymph vessels were 100∼150 μm diameters. Three different tissues of pure primo vessel, mixed primo+lymph vessel, and only lymph vessel were followed to RNA-Seq analysis by NGS. We also analyzed differentially expressed genes (DEG) from the RNA-Seq Data, in which thirty genes of the primo vessels, primo+lymph vessels, and lymph vessels were selected for primo marker candidates. From the plot of DEG analysis the ten genes were remarkably different expression pattern on the Group 1 (primo vessel) vs Group 3 (lymph vessel). With Fragments Per Kilobase Million (FPKM) the cutoff p-value for each gene was <0.05. FPKMs of the ten genes such as IGHM, HLA-DRA, HIST1H41, LPL, CD36, SRGN, DGAT2, SNCG, CD48, and GPD1 for primo vessels compared those of lymph vessels were increased double or triple times each other. These results suggest that the selected genes could be used for the specific marker to construct primo connectome of circuit system in rabbit.