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Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins.

Alexandra Rockstroh, Luisa Barzon, Widuranga Kumbukgolla, Hoang Xuan Su, Erley Lizarazo, Maria Fernanda Vincenti-Gonzalez, Adriana Tami, Alice M M Ornelas, Renato Santana Aguiar, Daniel Cadar, Jonas Schmidt-Chanasit, Sebastian Ulbert
Emerging Infectious Diseases 2019 January
We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings.

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