Regulating Cofactor Balance in vivo via Synthetic Flavin Analogue System.

A novel strategy to regulate cofactor balance in vivo for whole cell biotransformation using synthetic flavin analogue system was reported. High efficiency, easy operation, and good applicability were observed for the present system. Confocal laser scanning microscopy was employed to verify synthetic flavin analogue can directly permeate into Escherichia coli cells without modifying the cell membrane. This work provides a promising intracellular redox regulatory approach to construct more efficient cell factories.