Encapsulation of Bacillus salmalaya 139SI using Double Coating Bio-Polymer Technique.

Sustainable crop production for a rapidly growing human population is one of the current challenges faced by the agricultural sector. However, many of the chemical agents used in agriculture can be hazardous to humans, non-targeted organism and environment. Plant growth promoting rhizobacteria have demonstrated role in promoting plant growth and health under various stress conditions including disease. Unfortunately, bacteria viability degrades due to temperature and other environmental factors (Bashan et al. 2014). Encapsulation of bacteria into core-shell biopolymers is one of the promising techniques to overcome the problem. This study deals with the encapsulation of Bacillus salmalaya 139SI using simple double coating bio-polymer technique which consist of brown-rice protein/alginate and 0.5% low molecular weight chitosan of pH 4 and 6. The influence of bio-polymer to bacteria mass ratio and the pH of chitosan on the encapsulation process, physic-chemical, morphology and bioactivity properties of encapsulated Bacillus salmalaya 139SI have been studied systematically. Based on the analysis of physico-chemical, morphology and bioactivity properties, Bacillus salmalaya 139S1 encapsulated using double coating encapsulation technology has promising viability pre and post freeze-drying with excellent encapsulation yields of 99.7% and 89.3% respectively. This article is protected by copyright. All rights reserved.