Growth kinetics in Candida spp.: Differences between species and potential impact on antifungal susceptibility testing as described by the EUCAST.

We studied the growth kinetic parameters of clinically relevant Candida species to verify the differences between species following the incubation and medium conditions recommended by the EUCAST. We analyzed 705 susceptible Candida spp. from patients with candidemia and Candida glabrata isolates resistant to echinocandins or fluconazole (n = 38) and calculated the average growth rate, maximum peak, time to maximum rate, and lag phase. We also examined inter- and intra-species differences, as well as the percentage of isolates reaching an optical density of 0.2 over time. Interspecies differences in growth phases and kinetic parameters were found. C. glabrata was the fastest growing species and the lag phase of C. parapsilosis was longer than that of the other species considered in this study. Strain-to-strain variations were found between species. A positive correlation between the average growth rate and maximum peak was determined. Echinocandin-resistant C. glabrata isolates had significantly lower average growth rate but higher time to maximum rate in comparison to wild-type C. glabrata isolates. Incubation periods of 12-15 hours allowed reaching the 0.2 optical density threshold in 100% of C. glabrata, C. tropicalis, and C. krusei isolates. We show differences in kinetic parameters between Candida spp. C. glabrata was the fastest growing species and C. parapsilosis showed the longest lag phase. Resistance to echinocandins may affect the growth kinetic curve. Speeding up antifungal susceptibility results could be possible for some isolates, particularly C. glabrata, C. tropicalis, and C. krusei.