Correlation of apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G genetic variant rs8177832 with HIV-1 predisposition in Pakistani Population.

BACKGROUND: Human immunodeficiency virus (HIV) infection is a global health burden which ultimately results in acquired immune deficiency syndrome (AIDS). There are multiple host factors which are capable of limiting HIV-1 replication. One of the most important host factors which inhibit HIV-1 DNA synthesis is apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G (APOBEC3G). Any genetic variation of this important host factor may influence host susceptibility to viral infection.

OBJECTIVE: The aim of the current study was to evaluate any correlation of APOBEC3G genetic variation rs8177832 with HIV-1 infection.

METHODS: The study involved 142 healthy control and 100 HIV-1 infected subjects. The genetic variation rs8177832 of all studied subjects was determined by allele-specific polymerase chain reaction (AS-PCR).

RESULTS: The results showed that the distribution of rs8177832 genotypes AA, AG and GG in healthy subjects and HIV-1 subjects was; 42.253%, 42.957%, 14.788% and 66%, 27%, 7% respectively. Statistical analyses of data showed that there was significant variation of rs8177832 genotype AA in healthy control and HIV-1 infected subjects (42.257% vs 66%; p-value<0.001).

CONCLUSION: Thus it was concluded that APOBEC3G rs8177832 AA genotype is contributing in genetic predisposition to HIV-1 infection in Pakistani population.