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Multifunctional solid-state electrochemiluminescent chemosensors and aptasensor with free-standing active sites based on task-specific pyrene-terminated polymers via RAFT polymerization.
Analytica Chimica Acta 2018 December 19
Based on the flexible molecular engineering technique of reversible addition-fragmentation chain transfer (RAFT) polymerization, various polymers carrying positive or negative charges and different terminal groups such as pyrene or tertiary amine were synthesized for fabricating multifunctional solid-state electrochemiluminescent (ECL) sensors. Accordingly, the chemosensors immobilizing the ECL probe or co-immobilizing the ECL probe and the coreactant were realized for the quantification of small molecules (e.g., tripropylamine, tetracycline), and an aptasensor was developed for the specific and sensitive lysozyme assay (limit of detection: 0.1 ng/mL). All of the sensors were realized via a simple design exploiting the π-π stacking and electrostatic interactions. It was confirmed that the proposed strategy is simple but universal for the fabrication of versatile ECL sensors that showed simplicity, cost-effectiveness, high sensitivity, long-term stability, and excellent reproducibility.
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