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A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis.
Journal of Microbiological Methods 2018 October 12
We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its potential application in phenotypic pyrazinamide susceptibility testing.
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