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Synthesis of magnetic nanoparticle based molecularly imprinted polymer as a selective sorbent for efficient extraction of Ezetimibe from biological samples.
Biomedical Chromatography : BMC 2018 October 13
In the present study, a novel and efficient adsorbent constructed of molecularly imprinted polymer on the surface of modified magnetic nanoparticles with oleic acid (MNPs) was applied for the selective extraction of ezetimibe. The magnetic molecularly imprinted polymer (MMIP) was polymerized at the surface of modified MNPs using methacrylic acid (MAA) as functional monomer, ezetimibe as template and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The resulting MMIP showed high adsorption capacity, good selectivity and fast kinetic binding for the template molecule. It was characterized by Fourier transform infrared (FT-IR) analysis, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) methods. The maximum adsorption capacity of MMIP was obtained as 137.1 mg g-1 and it took about 20 minutes to achieve the equilibrium state. The adsorption model of the adsorbent was fitted with the Freundlich and Langmuir isotherm equations. The assay exhibited a linear range of 0.003-20.000 mg L-1 for ezetimibe with the correlation coefficient (R2 ) of 0.995. The relative standard deviations (RSDs) for the recoveries were less than 5.2. The method was also examined for the analysis of ezetimibe in the biological samples.
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