Model-based serial blood sampling protocol for minimal mortality and better recovery in small to medium sized tilapia.

Serial blood sampling involving sampling blood from the same individual at different time points is essential in time-based studies including xenobiotic toxicokinetics and biochemical studies. However, high fish mortality due to phlebotomy-induced anaemia (PIA) constrains serial blood sampling in small to medium sized fish. The aim of the study was to develop and implement a model-based serial blood sampling protocol that minimizes fish mortality by regulating anaemia within levels that sustain fish survival and recovery. A model simulating the reduction in haemoglobin was developed from blood sampling data of sixteen ( N =16) medium sized Oreochromis mossambicus The model was incorporated into a serial blood sampling protocol whose performance was tested on eight ( N =8) fish. The protocol avoided fish mortality and the fish recovered from PIA within three weeks of the post-sampling period. Therefore, managing anaemia minimizes mortality and improves the applicability of serial blood sampling in small to medium sized fish.