We have located links that may give you full text access.
Activated CaMKII α Binds to the mGlu 5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization.
Molecular Pharmacology 2018 December
Ca2+ /calmodulin-dependent protein kinase II (CaMKII) and metabotropic glutamate receptor 5 (mGlu5 ) are critical signaling molecules in synaptic plasticity and learning/memory. Here, we demonstrate that mGlu5 is present in CaMKII α complexes isolated from mouse forebrain. Further in vitro characterization showed that the membrane-proximal region of the C-terminal domain (CTD) of mGlu5a directly interacts with purified Thr286-autophosphorylated (activated) CaMKII α However, the binding of CaMKII α to this CTD fragment is reduced by the addition of excess Ca2+ /calmodulin or by additional CaMKII α autophosphorylation at non-Thr286 sites. Furthermore, in vitro binding of CaMKII α is dependent on a tribasic residue motif Lys-Arg-Arg (KRR) at residues 866-868 of the mGlu5a -CTD, and mutation of this motif decreases the coimmunoprecipitation of CaMKII α with full-length mGlu5a expressed in heterologous cells by about 50%. The KRR motif is required for two novel functional effects of coexpressing constitutively active CaMKII α with mGlu5a in heterologous cells. First, cell-surface biotinylation studies showed that CaMKII α increases the surface expression of mGlu5a Second, using Ca2+ fluorimetry and single-cell Ca2+ imaging, we found that CaMKII α reduces the initial peak of mGlu5a -mediated Ca2+ mobilization by about 25% while doubling the relative duration of the Ca2+ signal. These findings provide new insights into the physical and functional coupling of these key regulators of postsynaptic signaling.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app