Genetic Diversity and Drug Resistance of HIV-1 Circulating in North Sulawesi, Indonesia.

Manado, the capital city of North Sulawesi, is a unique region in Indonesia because of its religion. We collected peripheral blood samples from 63 individuals on antiretroviral therapy (ART). The amplification of viral genomic fragments, viral subtyping, detection of human immunodeficiency virus (HIV) drug resistance-associated mutations (DRAMs), and phylogenetic analyses were performed. Viral subtyping revealed that the most prevalent HIV type 1 (HIV-1) subtype/circulating recombinant form (CRF) was CRF01_AE (84.1%), followed by subtype B (6.8%) and recombinants between CRF01_AE and CRF02_AG (4.5%). Although no major DRAMs were present in PR genes, they were detected in RT genes. Nine out of 38 samples (23.7%) had major DRAMs against nucleoside RT inhibitors (NRTIs) and/or non NRTIs. The results of phylogenetic analyses indicated that CRF01_AE in North Sulawesi is related to that in Bali. Therefore, Bali may play an important role in circulating CRF01_AE in North Sulawesi.