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Journal Article
Research Support, Non-U.S. Gov't
Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon.
Biomolecules 2018 September 18
Congenital Pouch Colon (CPC) is a rare anorectal anomaly common to northwestern India, specifically Rajasthan. Despite efforts to understand the clinical genetic makeup of CPC, no attempt on identifying non-coding RNAs was done. We have earlier reported CPC's rare variants from whole exome sequencing (WES) across 18 affected samples in a total of 64 subjects. A Smith⁻Waterman algorithm was used to infer a couple of lncRNAs from WES samples of CPC with predictions from the Noncode database. Further screening and quantification using polymerase chain reaction (PCR), we ascertained interactions using Micro Scale Thermophoresis (MST). We report the role of lnc -EPB41-1-1 shown to be promiscuously interacting with KIF13A substantiating their role in regulation.
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