ENDOR Characterization of (N 2 )Fe II (μ-H) 2 Fe I (N 2 ) - : A Spectroscopic Model for N 2 Binding by the Di-μ-hydrido Nitrogenase Janus Intermediate.

The biomimetic diiron complex 4-(N2 )2 , featuring two terminally bound Fe-N2 centers bridged by two hydrides, serves as a model for two possible states along the pathway by which the enzyme nitrogenase reduces N2 . One is the Janus intermediate E4 (4H), which has accumulated 4[e-/H+], stored as two [Fe-H-Fe] bridging hydrides, and is activated to bind and reduce N2 through reductive elimination (RE) of the hydride ligands as H2 . The second is a possible RE intermediate. 1 H and 14 N 35 GHz ENDOR measurements confirm that the formally Fe(II)/Fe(I) 4-(N2 )2 complex exhibits a fully delocalized, Robin-Day type-III mixed valency. The two bridging hydrides exhibit a fully rhombic dipolar tensor form, T ≈ [- t, + t, 0]. The rhombic form is reproduced by a simple point-dipole model for dipolar interactions between a bridging hydride and its "anchor" Fe ions, confirming validity of this model and demonstrating that observation of a rhombic form is a convenient diagnostic signature for the identification of such core structures in biological centers such as nitrogenase. Furthermore, interpretation of the 1 H measurements with the anchor model maps the g tensor onto the molecular frame, an important function of these equations for application to nitrogenase. Analysis of the hyperfine and quadrupole coupling to the bound 14 N of N2 provides a reference for nitrogen-bound nitrogenase intermediates and is of chemical significance, as it gives a quantitative estimate of the amount of charge transferred between Fe and coordinated N, a key element in N2 activation for reduction.