CHROMOSOMAL INSTABILITY AS A CYTOGENETIC MARKER IN CHILDREN WITH VARYING DEGREES OF CONTROL OF ASTHMA.

The aim of investigation was to learn cytogenetic karyotype characteristics of children with asthma, with varying degrees of control in order to find markers of disease severity. The study involved 82 children aged 6 to 18 years, patients with asthma who were treated in Allergic Department of Regional Pediatric Hospital of Ivano-Frankivsk. Regarding the level of controlled asthma as a result of the application of test control asthma (GINA, 2011) children were distributed as follows: - 22 (30.6%) with controlled (CBA), 24 (33.3%) - is partly controlled (PCBA) 26 (36.1%) - with uncontrolled asthma (NCBA). The control group children were selected by random sampling, living in different parts of the Ivano-Frankivsk region (10 persons). Research of cytogenetic features of the karyotype in children with bronchial asthma was conducted by studying specimens of prometaphase chromosomes of peripheral blood lymphocytes. We analyzed associations of acrocentral number of chromosomes, karyotype features. The study involved 82 children aged 6 to 18 years with asthma of varying degrees of its control over the outcome of asthma control test. Children with uncontrolled asthma received significantly higher rate of chromosome acrocentral associations 13-15 (DD), 21-22 (GG) and 13-15 - 21-22 (DG). In patients with asthma we observed a lower mitotic activity compared with that in healthy ones (PN <0.05), and with a lower degree of controllability it decreased. Recognition of the genetic mechanisms of asthma leads to a new understanding of the genesis of the disease and can move forward in developing methods of treatment and prevention.