Dietary salmon milt extracts attenuate hepatosteatosis and liver dysfunction in diet-induced fatty liver model.

BACKGROUND: Dietary nucleotides have several reported beneficial effects. Here, we report a proteomic analysis of the effect of dietary nucleotides-rich salmon milt extract (SME) on the liver in a diet-induced fatty liver model.

RESULTS: Young male normal ddY mice were fed a normal chow diet, high carbohydrate diet (HCD), HCD containing 1% SME, or HCD containing 10% SME for 5 days following by a 2-day fast. Increased serum alanine transferase and aspartate transferase activities were observed in the HCD group and were significantly attenuated in the SME groups (P < 0.05). Hepatic steatosis was observed in all the HCD groups. Hepatic expression of Tnfα was significantly suppressed in the 10% SME group (P < 0.05). Comprehensive proteomic analysis of the liver in the SME groups revealed an increase in the levels of major proteins involved in mitochondrial bioenergetics, including peroxisome proliferator-activated receptor gamma coactivator 1 alpha, mitochondrial thioredoxin, cardiolipin synthase, peroxisome proliferator-activated receptor alpha, and carnitine palmitoyltransferase I.

CONCLUSION: Dietary SME improved liver function in the diet-induced fatty liver model. Activation of mitochondrial biogenetic function might be involved in this process. This article is protected by copyright. All rights reserved.