Comment on "establishment of human retinal mitoscriptome gene expression signature for diabetic retinopathy using cadaver eyes".

We read with great interest the article by Gowthaman and colleagues. The study's findings are useful for the discovery of biomarker and developing therapeutic regimen in diabetic retinopathy. On the other hand, from our perspective, the bioinformatics analyses need further context as the statistics for differential fold changes in expression data are not explained fully. Due to the high false positives caused by a large number of probes and multiple comparisons, it seems essential to analyze microarray data properly to reach a reliable result by a statistical method. Only selecting genes with >0.6-fold change in expression is not reliable and suitable for high-level microarray analysis.