We have located links that may give you full text access.
PPARγ/Pgc-1α-Fndc5 pathway up-regulation in gastrocnemius and heart muscle of exercised, branched chain amino acid diet fed mice.
Background: Previous studies have revealed the inductive effect of branched-chain amino acids (BCAAs) catabolism on fatty acid oxidation and metabolism, especially in muscle cells. In the present investigation, we have attempted to address whether a combination of BCAAs supplement consumption with aerobic exercise could elaborate the expression of PPAR γ, Pgc-1α and Fndc5 genes in gastrocnemius muscle and heart tissue of male C57BL/6 mice.
Methods: Thirty-six young male mice with an average weight of 18 ± 2 g were selected. Mice were randomly assigned to 6 groups: 20 mg/mL of BCAAs consumption with simultaneous exercise-training, 60 mg/mL of BCAAs consumption with simultaneous exercise-training, exercise-trained with no BCAAs consumption group, 20 mg/mL BCAAs without exercise-training, 60 mg/mL BCAAs without exercise-training, and untrained mice without BCAAs consumption.
Results: The findings showed a combination of 20 mg/mL BCAAs with aerobic exercise significantly increased Fndc5 , PPARγ , Pgc-1α gene expression in skeletal muscles although, circulating Irisin levels remained unchanged ( p < 0.05). Interestingly, plasma urea and lactate levels were significantly increased in 60 mg/mL BCAAs administrated mice which performed exercised ( p < 0.05). Two-way analysis of variance (ANOVA) was used to examine significant difference between groups and sedentary group.
Conclusions: Results showed inductive effect of 20 mg/mL BCAAs on expression levels of Fndc5 , PPARγ , Pgc-1α in gastrocnemius muscle similar with counterparts in heart tissue. Of note, higher serum irisin levels were detected after 20 mg/mL BCAAs supplementation coincided with the exercise.
Graphical abstract: An Overview on supplemantaion of branched chain amoinoacids on metablism of skeletal muscle and heart.
Methods: Thirty-six young male mice with an average weight of 18 ± 2 g were selected. Mice were randomly assigned to 6 groups: 20 mg/mL of BCAAs consumption with simultaneous exercise-training, 60 mg/mL of BCAAs consumption with simultaneous exercise-training, exercise-trained with no BCAAs consumption group, 20 mg/mL BCAAs without exercise-training, 60 mg/mL BCAAs without exercise-training, and untrained mice without BCAAs consumption.
Results: The findings showed a combination of 20 mg/mL BCAAs with aerobic exercise significantly increased Fndc5 , PPARγ , Pgc-1α gene expression in skeletal muscles although, circulating Irisin levels remained unchanged ( p < 0.05). Interestingly, plasma urea and lactate levels were significantly increased in 60 mg/mL BCAAs administrated mice which performed exercised ( p < 0.05). Two-way analysis of variance (ANOVA) was used to examine significant difference between groups and sedentary group.
Conclusions: Results showed inductive effect of 20 mg/mL BCAAs on expression levels of Fndc5 , PPARγ , Pgc-1α in gastrocnemius muscle similar with counterparts in heart tissue. Of note, higher serum irisin levels were detected after 20 mg/mL BCAAs supplementation coincided with the exercise.
Graphical abstract: An Overview on supplemantaion of branched chain amoinoacids on metablism of skeletal muscle and heart.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app