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Fabrication of poly (methyl methacrylate)/Ce/Cu substituted apatite/Egg white (Ovalbumin) biocomposite owning adjustable properties: Towards bone tissue rejuvenation.
A new biocomposite including Poly (methyl methacrylate)/Ce-Cu substituted apatite/Egg white (Ovalbumin) (PMMA/MHAP/EW) has been effectively manufactured via lyophilization technique. The reason to plan such biocomposite is to investigate for a perfect simple which may bio-imitate the constitution of natural bone for hard tissue designing regarding appropriate biodegradability, biocompatibility, and bactericidal properties. The FTIR and XRD spectra of prepared biocomposite showed the considerable intermolecular interface between the different constituents of the biocomposites. The examination of SEM pictures of the biocomposites demonstrated that the addition of EW affected the exterior morphology while a superior in-vitro biocompatibility has been seen in PMMA/MHAP/EW than in PMMA/MHAP in view of ALP, hemocompatibility and mineralized extracellular matrix studies, referring a more superior probability for assembly straight attach to living hard tissues if embedded. Additionally, cytocompatibility assay exposed the superior non-lethal character of prepared biocomposites to MG-63 cells when contrasted with PMMA/MHAP. The relative biodegradation studies of prepared biocomposites exposed a superior degradation rate for Comp-3. Besides, PMMA/MHAP/EW showed enhanced antibacterial property against both E. coli as well as S. aureus microbes with respect to PMMA/MHAP. These findings have set PMMA/MHAP/EW biocomposites on the platform to be utilized as a prospective option biomaterial for hard tissue designing.
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