miR-718 is involved in malignancy of papillary thyroid cancer through repression of PDPK1.

BACKGROUND: MicroRNAs bind the 3' untranslated regions (3'-UTRs) of mRNAs and thereby regulate gene expression post-transcriptionally and play an important role in cancer delvelopment. In the present study, we have explored the role of miR-718 in papillary thyroid cancer cell malignancy.

MATERIALS/METHODS: Here we examined the miRNA expression in human papillary thyroid cancer by RT-PCR. Luciferase activity, RT-PCR and western blot assays were used to confirmed the target of miRNA. MTT, colony formation, transwell, glucose consumption and lactate production assays were performed to analyze papillary thyroid cancer cell function. Western blot for signaling proteins was used to reveal the mechanism.

RESULTS: We first determined that miR-718 mRNA expression levels in PTC samples were reduced. The 3'-UTR of 3-Phosphoinositide Dependent Protein Kinase 1 (PDPK1) was then identified as a target of miR-718. Luciferase assays showed that miR-718 does in fact bind the wild-type PDPK1 3'-UTR. We assessed the effects of miR-718 on p-Akt, Akt, p-mTOR and mTOR expression. We determined that miR-718 negatively regulates their levels, respectively, of Akt-mTOR pathway components. We then assessed the effects of miR-718 on PTC cell behavior. The results revealed that miR-718 negatively regulates PTC cell proliferation, migration, and invasion. In addition, miR-718 was found to inhibit cell glucose metabolism, likely through the Akt-mTOR pathway. Finally, PDPK1 could rescue PTC cell inhibition induced by miR-718.

CONCLUSIONS: The present study strongly suggests that miR-718 inhibits PTC cell proliferation, metastasis, and glucose metabolism by negatively regulating the Akt-mTOR signaling pathway.