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Identification of a Novel Inhibitor of HRV Replication and Inflammation in Airway Epithelial Cells.
American Journal of Respiratory Cell and Molecular Biology 2018 August 30
Human rhinovirus (RV), the major cause of the common cold, triggers the majority of acute airway exacerbations in patients with asthma and chronic obstructive pulmonary disease. Nitric oxide (NO), and the related metabolite S-nitrosoglutathione (GSNO), are produced in the airway epithelium via nitric oxide synthase 2 (NOS2) and have been shown to function in host defense against RV infection. We hypothesized that inhibitors of the GSNO-metabolizing enzyme, GSNO reductase (GSNOR), might potentiate the antiviral properties of airway-derived NOS2. Using in vitro models of RV-A serotype 16 (RV-A16) and mNeonGreen-H1N1pr8 infection of human airway epithelial cells, we found that treatment with a previously-characterized GSNOR inhibitor (4-[[2-[[(3-cyanophenyl)methyl]thio]-4-oxothieno-[3,2-d]pyrimidin-3(4H)-yl]methyl]-benzoic acid; referred to as C3m) decreased RV-A16 replication and expression of downstream pro-inflammatory and antiviral mediators (e.g. RANTES, CXCL10 and Mx1), and increased nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent genes (e.g. SQSTM1 and TrxR1). In contrast, C3m had no effect on influenza virus H1N1pr8 replication. Moreover, a structurally dissimilar GSNOR inhibitor (N6022) did not alter RV replication, suggesting that the properties of C3m may be specific to rhinovirus due to an off-target effect. Consistent with this, C3m antiviral effects were not blocked by either NOS inhibition or GSNOR knockdown but appeared to be mediated by reduced ICAM-1 transcription and increased shedding of soluble ICAM-1 protein. Collectively these data show that C3m has novel anti-rhinoviral properties that may synergize with, but are unrelated to, its GSNOR inhibitor activity.
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