We have located links that may give you full text access.
Cadmium induces ovarian granulosa cell damage by activating pERK-eIF2α-ATF4 through endoplasmic reticulum (ER) stress†.
Biology of Reproduction 2018 August 19
ABSTRCT: This study aimed to investigate whether cadmium induces ovarian granulosa cell damage by activating PERK-eIF2α-ATF4 through endoplasmic reticulum (ER) stress and to elucidate the underlying regulation mechanism. Two models of cadmium exposure were established. In one model, ovarian granulosa cells isolated from 21-day-old female Sprague Dawley (SD) rats were cultured in vitro for 36 h and exposed to CdCl2 (0, 5, 10, and 20 μM), and in another model, a human ovarian granulosa tumor cell line (COV434) was used to construct the BIP- knockdown cell line sh-BIP and exposed to 0 and 20 μM CdCl2. after exposure to cadmium for 12 h, the expression mRNA and protein levels of BIP, p-PERK and p-eIF2α were determined in the two models. miRNAs related to BIP were also detected in granulosa cells after cadmium exposure. We found that mRNA and protein levels of all factors were up-regulated in each cadmium-dose group, except for BIP mRNA expression in the 5 μM Cd group. The BIP gene was knocked down in COV434 cells before exposure to cadmium. All factors were up-regulated in COV434 cells exposed to Cd, and the expression of the p-eIF2α protein was down-regulated in sh-BIP cells exposed to Cd. In addition, no differences in BIP related miRNAs were detected in cadmium-exposed rat ovarian granulosa cells versus the control group. Cadmium induces ovarian granulosa cell damage by inducing ER stress.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
Perioperative echocardiographic strain analysis: what anesthesiologists should know.Canadian Journal of Anaesthesia 2024 April 11
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app