Phytotoxicity and bioaccumulation of zinc oxide nanoparticles in rice (Oryza sativa L.).

This work focused on the toxicity evaluation of ZnO NPs and their uptake and transportation in a significant crop plant, Rice (Oryza sativa L.). Under hydroponic condition, 25, 50 and 100 mg/L ZnO NPs could inhibit the growth of rice seedlings by reducing their biomass comparing with Zn2+ (13.82 mg/L) treatment and the control. In addition, physiological index was determined, involving the decrease of the chlorophyll content, which was further confirmed by the down-regulation of photosynthetic pigment related genes. Based on the expression levels of the genes encoding three antioxidant enzyme, e.g. Catalase (EC 1.11.1.6), Ascorbate peroxidase (EC 1.11.1.11) and Superoxide dismutase (EC 1.15.1.1), the oxidative damage was found in ZnO NPs exposed rice. On the other hand, by ultra-thin slicing and transmission electron microscopy, ZnO NPs were observed in the intercellular space and cytoplasm of rice root cells, and their transport to aerial tissue from roots were further confirmed by inductively coupled plasma atomic emission spectrometer. Overall, ZnO NPs could be uptaken by rice in the form of ions or particles, which further affected plant growth and development at phenotypic, physiological and molecular levels.